PFKFB3 antibody (AA 412-520)

Catalog No. ABIN518808

Cited in 3+ publications. The Mouse Monoclonal anti-PFKFB3 antibody (Clone 3F3) (ABIN518808) specifically detects PFKFB3 in WB, ELISA and IP. The antibody is reactive with Human samples.

Quick Overview for PFKFB3 antibody (AA 412-520) (ABIN518808)

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PFKFB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 3F3

Product Details anti-PFKFB3 Antibody

Binding Specificity: AA 412-520

Purpose: Mouse monoclonal antibody raised against a partial recombinant PFKFB3.

Sequence: CPLHTVLKLT PVAYGCRVES IYLNVESVCT HRERSEDAKK GPNPLMRRNS VTPLASPEPT KKPRINSFEE HVASTSAALP SCLPPEVPTQ LPGQNMKGSR SSADSSRKH

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PFKFB3 (NP_004557, 412 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PFKFB3 antibody (ABIN518808)

Ohmura, Hishiki, Yamamoto, Nakanishi, Kubo, Tsuchihashi, Tamada, Toue, Kabe, Saya, Suematsu: "Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 46, pp. 102-13, (2015) (PubMed).

Yamamoto, Takano, Ishiwata, Ohmura, Nagahata, Matsuura, Kamata, Sakamoto, Nakanishi, Kubo, Hishiki, Suematsu: "Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway." in: Nature communications, Vol. 5, pp. 3480, (2014) (PubMed).

Bao, Mukai, Hishiki, Kubo, Ohmura, Sugiura, Matsuura, Nagahata, Hayakawa, Yamamoto, Fukuda, Saya, Suematsu, Minamishima: "Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo." in: Molecular cancer research : MCR, Vol. 11, Issue 9, pp. 973-85, (2013) (PubMed).

Target Details for PFKFB3

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Alternative Name: PFKFB3

Background: Full Gene Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Synonyms: IPFK2,PFK2

Gene ID: 5209

NCBI Accession: NM_004566

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process