SMT1 antibody

Catalog No. ABIN5326722

This Rabbit Polyclonal antibody specifically detects SMT1 in IL and WB. It exhibits reactivity toward Arabidopsis thaliana. It has been mentioned in 2+ publications

Quick Overview for SMT1 antibody (ABIN5326722)

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details anti-SMT1 Antibody

Purpose: Anti-SMT1 | Sterol methyltransferase 1

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Triticum aestivum, Solanum lycopersicum, Withania somnifera

Predicted Reactivity: Amborella trichopoda,Brassica napus,Brassica rapa,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium mexicanum,Medicago truncatula,Populus trichopocarpa,Prunus persica,Ricinus communis,Theobroma cacao,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02

Application Details

Application Notes: 1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Comment:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-SMT1 antibody (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Target Details for SMT1

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Alternative Name: Sterol methyltransferase 1 (SMT1)

Background:

Background: SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Additional information: SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.

Molecular Weight: 38 kDa

Gene ID: 831216

UniProt: Q9LM02