RAD50 antibody (AA 1-425)

Catalog No. ABIN2627482

The Mouse Monoclonal anti-RAD50 antibody (Clone 13B3) (ABIN2627482) specifically detects RAD50 in WB, IHC, IP, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for RAD50 antibody (AA 1-425) (ABIN2627482)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 13B3

Product Details anti-RAD50 Antibody

Binding Specificity: AA 1-425

Specificity: Amino acids 1-425 of Rad50 expressed in E. coli.

Purification: Protein G purified

Immunogen: Recombinant human RAD50.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50

Background: Name/Gene ID: RAD50

Synonyms: RAD50, DNA repair protein RAD50, HRad50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene ID: 10111

Pathways: DNA Damage Repair, Protein targeting to Nucleus