CRYBA1 antibody (C-Term)

Catalog No. ABIN5514391

The Rabbit Polyclonal anti-CRYBA1 antibody is suitable to detect CRYBA1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for CRYBA1 antibody (C-Term) (ABIN5514391)

Target: CRYBA1 (Crystallin, beta A1 (CRYBA1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYBA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRYBA1 Antibody

Binding Specificity: C-Term

Purpose: CRYBA1 Antibody - C-terminal region

Sequence: SGAWVCYQYP GYRGYQYILE CDHHGGDYKH WREWGSHAQT SQIQSIRRIQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CRBA1. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human CRBA1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52062-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CRYBA1

Target: CRYBA1 (Crystallin, beta A1 (CRYBA1))

Alternative Name: CRYBA1

Background:

Background Information: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Gene Name: crystallin beta A1

Alternative Symbols: CRYB1, CTRCT10

Protein Name: beta-crystallin A3

Molecular Weight: 23kDa

Gene ID: 1411

NCBI Accession: NP_005199

UniProt: P05813