PFKFB3 antibody (C-Term)

Catalog No. ABIN5514397

The Rabbit Polyclonal anti-PFKFB3 antibody is suitable to detect PFKFB3 in samples from Human, Rat, Mouse, Guinea Pig, Cow, Horse, Rabbit and Dog. It has been validated for WB.

Quick Overview for PFKFB3 antibody (C-Term) (ABIN5514397)

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Reactivity: Human, Rat, Mouse, Guinea Pig, Cow, Horse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKFB3 Antibody

Binding Specificity: C-Term

Purpose: PFKFB3 Antibody - C-terminal region

Sequence: VESVCTHRER SEDAKKGPNP LMRRNSVTPL ASPEPTKKPR INSFEEHVAS

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against F263. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human F263

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56582-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PFKFB3

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Alternative Name: PFKFB3

Background:

Background Information: The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Alternative Symbols: PFK2, IPFK2, iPFK-2

Protein Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3

Molecular Weight: 57kDa

Gene ID: 5209

UniProt: Q16875

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process