ANAPC5 antibody (Middle Region)

Catalog No. ABIN5514501

This Rabbit Polyclonal antibody specifically detects ANAPC5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow and Zebrafish (Danio rerio).

Quick Overview for ANAPC5 antibody (Middle Region) (ABIN5514501)

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ANAPC5 Antibody

Binding Specificity: Middle Region

Sequence: HSLLHYFDRL ILTGAESKSN GEEGYGRSLR YAALNLAALH CRFGHYTSPP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ANAPC5. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human ANAPC5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ANAPC5

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Alternative Name: ANAPC5

Background: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Alias Symbols: ANAPC5, APC5,

Protein Interaction Partner: ANAPC2, UBE2S, UBC, ANAPC4, HECW2, LATS2, PHF8, SOX2, PTEN, CDC27, BACH1, TRIM33, ANAPC7, ANAPC15, CDH1, CDC20, PAXIP1, MDC1, TP53BP1, NOTCH1, ANAPC1, ANAPC13, CDC16, CDC23, FBXO5, USP37, CUL4A, SH3KBP1, E2F1, SMARCAD1, MAPK6, Gm9174, Anapc5, Mad2l1, Anap

Protein Size: 219

Gene ID: 51433

Pathways: M Phase