ANAPC5 antibody (AA 120-200)

Catalog No. ABIN7213622

The Rabbit Polyclonal anti-ANAPC5 antibody is suitable to detect ANAPC5 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for ANAPC5 antibody (AA 120-200) (ABIN7213622)

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ANAPC5 Antibody

Binding Specificity: AA 120-200

Purpose: Rabbit Anti-APC5 Polyclonal Antibody

Specificity: APC5 Polyclonal Antibody detects endogenous levels of APC5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human APC5 at AA range: 120-200

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ANAPC5

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Alternative Name: APC5

Background: ANAPC5, APC5, Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5ANAPC5 encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein (anaphase promoting complex subunit 5) is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Molecular Weight: 85kD

Gene ID: 51433

UniProt: Q9UJX4

Pathways: M Phase