PKM antibody (C-Term)

Catalog No. ABIN5514803

The Rabbit Polyclonal anti-PKM antibody (ABIN5514803) specifically detects PKM in WB. The antibody is reactive with Human samples.

Quick Overview for PKM antibody (C-Term) (ABIN5514803)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKM Antibody

Binding Specificity: C-Term

Purpose: PKM Antibody - C-terminal region

Sequence: VICATQMLES MIKKPRPTRA EGSDVANAVL DGADCIMLSG ETAKGDYPLE

Characteristics: This is a rabbit polyclonal antibody against KPYM. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human KPYM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP75032-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background:

Background Information: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Gene Name: pyruvate kinase M1/2

Alternative Symbols: PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1, HEL-S-30

Protein Name: pyruvate kinase PKM

Molecular Weight: 58kDa

Gene ID: 5315

NCBI Accession: XP_005254502

UniProt: P14618

Pathways: Warburg Effect