Histone H3.3 antibody (N-Term)

Catalog No. ABIN5514837

This Rabbit Polyclonal antibody specifically detects Histone H3.3 in WB. It exhibits reactivity toward Human.

Quick Overview for Histone H3.3 antibody (N-Term) (ABIN5514837)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H3.3 Antibody

Binding Specificity: N-Term

Purpose: H3F3A Antibody - N-terminal region

Sequence: RTKQTARKST GGKAPRKQLA TKAARKSAPS TGGVKKPHRY RPGTVALREI

Characteristics: This is a rabbit polyclonal antibody against H3F3A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human H3F3A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP66181-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3F3A

Background:

Background Information: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Alternative Symbols: H3F3, H3-3B, H3.3A, H3F3A

Molecular Weight: 14kDa

Gene ID: 3020

NCBI Accession: NP_005315

UniProt: P84243