ROBO3 antibody (C-Term)

Catalog No. ABIN5515113

This Rabbit Polyclonal antibody specifically detects ROBO3 in WB. It exhibits reactivity toward Human.

Quick Overview for ROBO3 antibody (C-Term) (ABIN5515113)

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ROBO3 Antibody

Binding Specificity: C-Term

Purpose: ROBO3 Antibody - C-terminal region

Sequence: HPSRSPSAQE PRGSCCPSNP DPDDRYYNEA GISLYLAQTA RGTAAPGEGP

Characteristics: This is a rabbit polyclonal antibody against ROBO3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human ROBO3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP74184-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3

Background:

Background Information: This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.

Alternative Symbols: HGPS, RIG1, HGPPS, RBIG1, HGPPS1

Molecular Weight: 152kDa

Gene ID: 64221

NCBI Accession: NP_071765

UniProt: Q96MS0