RhoF antibody (N-Term)

Catalog No. ABIN5516321

The Rabbit Polyclonal anti-RhoF antibody (ABIN5516321) specifically detects RhoF in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae samples.

Quick Overview for RhoF antibody (N-Term) (ABIN5516321)

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RhoF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RhoF Antibody

Binding Specificity: N-Term

Purpose: RHOF Antibody - N-terminal region

Sequence: DGGCGKTSLL MVYSQGSFPE HYAPSVFEKY TASVTVGSKE VTLNLYDTAG

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Yeast: 79%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against RHOF. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human RHOF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57309-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RhoF

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Alternative Name: RHOF

Background:

Background Information: RHOF is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state.It causes the formation of thin, actin-rich surface projections called filopodia. And it functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin- based morphology.

Gene Name: ras homolog family member F (in filopodia)

Alternative Symbols: RIF, ARHF

Protein Name: Rho-related GTP-binding protein RhoF

Molecular Weight: 23kDa

Gene ID: 54509

NCBI Accession: NP_061907

UniProt: Q9HBH0