GRP78 antibody (C-Term)

Catalog No. ABIN5516335

The Rabbit Polyclonal anti-GRP78 antibody has been validated for WB and IHC. It is suitable to detect GRP78 in samples from Human, Rat, Mouse, Cow, Rabbit, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse and Goat.

Quick Overview for GRP78 antibody (C-Term) (ABIN5516335)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRP78 Antibody

Binding Specificity: C-Term

Sequence: LSSEDKETME KAVEEKIEWL ESHQDADIED FKAKKKELEE IVQPIISKLY

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against GRP78. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human GRP78

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: GRP78

Background: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Alias Symbols: HSPA5, GRP78,

Protein Interaction Partner: ERLIN2, HUWE1, GPX7, FUS, EP300, AMFR, UBC, RELA, LGALS3BP, AIRE, AURKB, CEP70, CEP76, HAUS2, TUBGCP4, CEP250, MAGED2, TUBGCP2, TUBGCP3, CEP57, TUBG1, TP53, AURKA, SUMO2, SUMO3, CLU, CDKN1A, CDC20, STAU1, SUMO1, NEDD8, MDM2, RPA3, RPA2, RPA1, EED, HSPA1L,

Protein Size: 654

Gene ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling