EIF5A antibody (N-Term)

Catalog No. ABIN5516707

The Rabbit Polyclonal anti-EIF5A antibody is suitable to detect EIF5A in samples from Human, Mouse, Rat, Cow, Rabbit, Sheep, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for EIF5A antibody (N-Term) (ABIN5516707)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF5A Antibody

Binding Specificity: N-Term

Sequence: LKGRPCKIVE MSTSKTGKHG HAKVHLVGID IFTGKKYEDI CPSTHNMDVP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF5A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human EIF5A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: EIF5A

Background: EIF5A is a mRNA-binding protein involved in translation elongation. It has an important function at the level of mRNA turnover, probably acting downstream of decapping. It is involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, EIF5A functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. EIF5A regulates also TNF-alpha-mediated apoptosis and mediates effects of polyamines on neuronal process extension and survival. It may play an important role in brain development and function, and in skeletal muscle stem cell differentiation. EIF5A is also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

Protein Interaction Partner: HUWE1, UBC, REL, MEOX2, DHPS, CRX, DCP2, STUB1, BCCIP, WDR1, GDA, TYMS, PEPD, MVD, HSPD1, GNS, GLS, UBD, NPM1, IQCB1, ATF2, VCP, MACF1, DDX17, VAPA, XPO1, ATP5B, XPO4, CUL2, COPS5, TCF3, ELAVL1, SUMO2, VHL, Pds5a, Csnk1e, DOHH, XRN2, RPL5, TGM2,

Protein Size: 184

Gene ID: 1984

Pathways: Regulation of Muscle Cell Differentiation