SETD7 antibody (N-Term)

Catalog No. ABIN5517321

Product Details anti-SETD7 Antibody

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD7 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SETD7. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETD7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SETD7

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Alternative Name: SETD7 (SETD7 Products)

Background: SETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

Alias Symbols: SETD7, KIAA1717, KMT7, SET7, SET9,

Protein Interaction Partner: KLF4, HIST3H3, SOX2, PRKAR1A, A1BG, TP53, NR2F2, STEAP3, SERTAD1, DPY30, MED1, MYOD1, E2F1, HIST1H3A, SMARCA4, H3F3A, STAT3, RELA, AR, UBC, DNMT1, PPP1R12A, SIRT1, TBP, TAF10, TAF7, TAF6, HIST1H4A, ESR1, HIST2H3C, HCFC1, L3MBTL1,

Protein Size: 366

Gene ID: 80854

NCBI Accession: NP_085151

UniProt: Q8WTS6