SETD7 antibody (N-Term)

Catalog No. ABIN5517321

This Rabbit Polyclonal antibody specifically detects SETD7 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for SETD7 antibody (N-Term) (ABIN5517321)

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SETD7 Antibody

Binding Specificity: N-Term

Sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SETD7. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETD7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SETD7

Target: SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))

Alternative Name: SETD7

Background: SETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

Alias Symbols: SETD7, KIAA1717, KMT7, SET7, SET9,

Protein Interaction Partner: KLF4, HIST3H3, SOX2, PRKAR1A, A1BG, TP53, NR2F2, STEAP3, SERTAD1, DPY30, MED1, MYOD1, E2F1, HIST1H3A, SMARCA4, H3F3A, STAT3, RELA, AR, UBC, DNMT1, PPP1R12A, SIRT1, TBP, TAF10, TAF7, TAF6, HIST1H4A, ESR1, HIST2H3C, HCFC1, L3MBTL1,

Protein Size: 366

Gene ID: 80854

NCBI Accession: NP_085151

UniProt: Q8WTS6