RSPO1 antibody (C-Term)

Catalog No. ABIN5517492

This Rabbit Polyclonal antibody specifically detects RSPO1 in WB. It exhibits reactivity toward Human.

Quick Overview for RSPO1 antibody (C-Term) (ABIN5517492)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RSPO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RSPO1 Antibody

Binding Specificity: C-Term

Purpose: RSPO1 Antibody - C-terminal region

Sequence: RCTVRRVPCP EGQKRRKGGQ GRRENANRNL ARKESKEAGA GSRRRKGQQQ

Predicted Reactivity: Cow: 85%, Dog: 92%, Goat: 85%, Horse: 92%, Human: 100%, Rabbit: 86%

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RSPO1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP64132-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RSPO1

Target: RSPO1 (R-Spondin 1 (RSPO1))

Alternative Name: RSPO1

Background:

Background Information: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.

Gene Name: R-spondin 1

Alternative Symbols: RSPO, CRISTIN3

Protein Name: R-spondin-1

Molecular Weight: 25 kDa

Gene ID: 284654

NCBI Accession: NP_001229838

UniProt: Q2MKA7