POLR2J3 antibody (N-Term)

Catalog No. ABIN5517606

This Rabbit Polyclonal antibody specifically detects POLR2J3 in WB. It exhibits reactivity toward Human.

Quick Overview for POLR2J3 antibody (N-Term) (ABIN5517606)

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2J3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2J3 Antibody

Binding Specificity: N-Term

Purpose: POLR2J3 Antibody - N-terminal region

Sequence: APPAFESFLL FEGEKITINK DTKVPNACLF TINKEDHTLG NIIKSQLLKD

Characteristics: This is a rabbit polyclonal antibody against POLR2J2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human POLR2J2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP69943-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR2J3

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Alternative Name: POLR2J3

Background:

Background Information: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.

Gene Name: RNA polymerase II subunit J3

Alternative Symbols: POLR2J2, RPB11b1, RPB11b2

Protein Name: DNA-directed RNA polymerase II subunit RPB11-b2

Molecular Weight: 12kDa

Gene ID: 548644

NCBI Accession: NP_001091084

UniProt: Q9GZM3