SIM2 antibody (Middle Region)

Catalog No. ABIN5517795

This Rabbit Polyclonal antibody specifically detects SIM2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow and Pig.

Quick Overview for SIM2 antibody (Middle Region) (ABIN5517795)

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIM2 Antibody

Binding Specificity: Middle Region

Purpose: SIM2 Antibody - middle region

Sequence: VLTEIEYKEL QLSLEQVSTA KSQDSWRTAL STSQETRKLV KPKNTKMKTK

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 91%, Pig: 93%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SIM2. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human SIM2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33297-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIM2

Target: SIM2 (Single-Minded Homolog 2 (SIM2))

Alternative Name: SIM2

Background:

Background Information: This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Gene Name: single-minded homolog 2 (Drosophila)

Alternative Symbols: SIM, bHLHe15, HMC13F06, HMC29C01

Protein Name: Single-minded homolog 2

Molecular Weight: 73kDa

Gene ID: 6493

UniProt: Q14190