CD40 antibody (N-Term)

Catalog No. ABIN5518644

This Rabbit Polyclonal antibody specifically detects CD40 in FACS and WB. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for CD40 antibody (N-Term) (ABIN5518644)

Target: CD40

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Product Details anti-CD40 Antibody

Binding Specificity: N-Term

Purpose: Anti-CD40 Antibody

Sequence: PEPPTACREK QYLINSQC

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD40 Antibody-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD40.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD40 antibody (ABIN5518644)

Meng, Li, Geng, Ohe, Yu, E, Ye, Yang, Kato, Zhang, Ishida, Ohta, Jin, Kakehata, Geng, Yamakawa: "Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy." in: Translational research : the journal of laboratory and clinical medicine, Vol. 166, Issue 3, pp. 281-91, (2015) (PubMed).

Peng, Cheng, Zeng, Shi, Bao, Mao, Ji, Li, Guo, Liang, Wu: "The role of RANTES as a crucial downstream cytokine in calcineurin-dependent VSMC apoptosis stimulated by INFgamma and CD40L." in: Cell biology international, Vol. 34, Issue 5, pp. 447-53, (2010) (PubMed).

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background:

Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

Gene Full Name: CD40 Molecule

Sequence Similarities: Contains 4 TNFR-Cys repeats.

Molecular Weight: 40 kDa

Gene ID: 958

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints