EPAS1 antibody (Middle Region)

Catalog No. ABIN5518650

This Rabbit Polyclonal antibody specifically detects EPAS1 in WB. It exhibits reactivity toward Human, Rat and Mouseand has been mentioned in 1 publication.

Quick Overview for EPAS1 antibody (Middle Region) (ABIN5518650)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 282-301, Middle Region

Purpose: Anti-HIF-2-alpha/EPAS1 Antibody Picoband®

Sequence: HALDSENMTK SHQNLCTKGQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HIF-2-alpha/EPAS1 Antibody (ABIN5518650). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Tian, H., McKnight, S. L., Russell, D. W. : Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. Genes Dev. 11: 72-82, 1997. 2. Sood, R., Zehnder, J. L., Druzin, M. L., Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006. 3. Henderson, J., Withford-Cave, J. M., Duffy, D. L., Cole, S. J., Sawyer, N. A., Gulbin, J. P., Hahn, A., Trent, R. J., Yu, B. : The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletes. Hum. Genet. 118: 416-423, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-EPAS1 antibody (ABIN5518650)

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1

Background:

Synonyms: Endothelial PAS domain-containing protein 1,EPAS-1,Hypoxia-inducible factor 2-alpha,HIF-2-alpha,HIF2-alpha,Epas1,Hif2a,

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molecular Weight: 120 kDa

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect