CD31 antibody (C-Term)

Catalog No. ABIN5518694

Cited in 37+ publications. The Rabbit Polyclonal anti-CD31 antibody (ABIN5518694) specifically detects CD31 in FACS, WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for CD31 antibody (C-Term) (ABIN5518694)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD31 Antibody

Binding Specificity: C-Term

Purpose: Anti-CD31/PECAM1 Antibody

Sequence: RKAVPDAVES RYSRTE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD31/PECAM1 Antibody-1. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD31.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD31 antibody (ABIN5518694)

Chen, Li, Peng, Jie, Dongye, Cai, Feng, Li, Zeng, Lun, Chen, Xu: "The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex." in: Oncotarget, Vol. 7, Issue 49, pp. 80716-80734, (2018) (PubMed).

Zhang, Peng, Yuan, Xie, Yang, Xue: "Peroxisome proliferator‑activated receptor γ mediates porcine placental angiogenesis through hypoxia inducible factor‑, vascular endothelial growth factor‑ and angiopoietin‑mediated signaling." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 2636-2644, (2018) (PubMed).

Fan, Qu, Chu, Gao, Gao, Chen, Tian: "MicroRNA-210 promotes angiogenesis in acute myocardial infarction." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5658-5665, (2018) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." in: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

Zhou, He, Liu, Feng, Zhang, Hua, Tang: "Angiogenic gene-modified myoblasts promote vascularization during repair of skeletal muscle defects." in: Journal of tissue engineering and regenerative medicine, Vol. 9, Issue 12, pp. 1404-16, (2016) (PubMed).

Chen, Yu, Gumireddy, Li, Yao, Gao, Chen, Hao, Wang, Huang, Xu, Ye: "ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer." in: Oncotarget, Vol. 6, Issue 9, pp. 6959-76, (2016) (PubMed).

Wang, Yang, Tang, Long, Huang: "Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling." in: Stem cells international, Vol. 2016, pp. 3269267, (2016) (PubMed).

Zhang, Wang, Yang, Pan, Li, Yuan: "Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing." in: Oncology letters, Vol. 11, Issue 5, pp. 3265-3272, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

Li, Zeng, Qi, Tang, Zhang, Wu, Liang, Shi, Liu, Zhang: "Xenotransplantation of human adipose-derived stem cells in zebrafish embryos." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123264, (2015) (PubMed).

Wang, Yin, Dong, Zhao, Wang, Wang, Wang, Du, Lu: "Inhibition of Janus kinase-2 signalling pathway ameliorates portal hypertensive syndrome in partial portal hypertensive and liver cirrhosis rats." in: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, Vol. 47, Issue 4, pp. 315-23, (2015) (PubMed).

Xu, Wang: "Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel." in: Biotechnology and bioengineering, Vol. 112, Issue 8, pp. 1683-95, (2015) (PubMed).

Xiao, Qiu, Cui, Ni, Li, Liao, Lu, Ding: "MicroRNA-885-3p inhibits the growth of HT-29 colon cancer cell xenografts by disrupting angiogenesis via targeting BMPR1A and blocking BMP/Smad/Id1 signaling." in: Oncogene, Vol. 34, Issue 15, pp. 1968-78, (2015) (PubMed).

Huan, Wang, Yang, Cui, Wen, Chen, Chen: "Expression and function of the ID1 gene during transforming growth factor-?1-induced differentiation of human embryonic stem cells to endothelial cells." in: Cellular reprogramming, Vol. 17, Issue 1, pp. 59-68, (2015) (PubMed).

Liu, Wang, Ye, Yin, Chen: "Distinct molecular basis for endothelial differentiation: gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells." in: Cellular immunology, Vol. 289, Issue 1-2, pp. 7-14, (2014) (PubMed).

Lu, Liu, Zhu, Xu: "Expression of nerve growth factor and hypoxia inducible factor-1? and its correlation with angiogenesis in non-small cell lung cancer." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 359-62, (2014) (PubMed).

Wang, Jiang, Wang, Li, Zhang, Zhao, Peng, Cao, Sun: "Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 413-418, (2014) (PubMed).

Wang, Yang, Wen, Ma, Wang, Yu, Tang, Wu, Dong, Cheng, Nie, Chen: "SKLB-M8 induces apoptosis through the AKT/mTOR signaling pathway in melanoma models and inhibits angiogenesis with decrease of ERK1/2 phosphorylation." in: Journal of pharmacological sciences, Vol. 126, Issue 3, pp. 198-207, (2014) (PubMed).

Xiu, Fan, Li, Cui, Lei: "Different angiogenic abilities of self-setting calcium phosphate cement scaffolds consisting of different proportions of fibrin glue." in: BioMed research international, Vol. 2014, pp. 785146, (2014) (PubMed).

Chen, Wan, Xia, Guo, Wang, Liu, Li: "Promoted regeneration of mature blood vessels by electrospun fibers with loaded multiple pDNA-calcium phosphate nanoparticles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fu?r Pharmazeutische Verfahrenstechnik e.V, Vol. 85, Issue 3 Pt A, pp. 699-710, (2013) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM1

Background:

Background: PECAM-1 (Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31 (CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al. (1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller (1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Gene Full Name: platelet and endothelial cell adhesion molecule 1

Sequence Similarities: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Molecular Weight: 120 kDa

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization