ITGAL antibody (AA 161-349)

Catalog No. ABIN5518766

Product Details anti-ITGAL Antibody

Target: ITGAL (Integrin alpha-L (ITGAL))

Binding Specificity: AA 161-349

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Integrin alpha-L(ITGAL) detection. Tested with WB, FCM in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Integrin alpha-L(ITGAL) detection. Tested with WB, FCM in Human.
Gene Name: integrin subunit alpha L
Protein Name: Integrin alpha-L

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Flow Cytometry: Concentration: 1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-ITGAL antibody (ABIN5518766)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: ITGAL (ITGAL Products)

Synonyms: CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody

Background: CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.

Synonyms: Antigen CD11A | CD 11a | CD11 antigen-like family member A | CD11a antigen | Integrin alphaL | Integrin alpha-L | ITAL | ITGAL protein | LFA 1A | LFA1A | LFA-1A | P20701

Gene ID: 3683

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex