PD-1 antibody (N-Term)

Catalog No. ABIN5518784

The Rabbit Polyclonal anti-PD-1 antibody (ABIN5518784) specifically detects PD-1 in FACS, IHC, WB and IF. The antibody is reactive with Mouse and Rat samples.

Quick Overview for PD-1 antibody (N-Term) (ABIN5518784)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PD-1 Antibody

Binding Specificity: N-Term

Purpose: Anti-PD-1/PDCD1 Antibody

Sequence: NGLSQPVQDA RFQIIQLPNR HDFHMNILDT RRND

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related human sequence by fifteen amino acids.

Characteristics: Anti-PD-1 PDCD1 Antibody. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse PD1, different from the related human sequence by fifteen amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1

Background:

Background: Transcription factor SOX-9 is a protein that in humans is encoded by the SOX9 gene. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Gene Full Name: programmed cell death 1

Molecular Weight: 60 kDa

Gene ID: 18566

UniProt: Q02242

Pathways: Cancer Immune Checkpoints