PDE5A antibody (N-Term)

Catalog No. ABIN5518785

This Rabbit Polyclonal antibody specifically detects PDE5A in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for PDE5A antibody (N-Term) (ABIN5518785)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDE5A Antibody

Binding Specificity: AA 20-63, N-Term

Purpose: Anti-PDE5A Antibody

Sequence: QKQQQRDQDS VEAWLDDHWD FTFSYFVRKA TREMVNAWFA ERVH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PDE5A Antibody (ABIN5518785). Tested in IHC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PDE5A, different from the related mouse sequence by eight amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: PDE5A

Background:

Synonyms: cGMP-specific 3',5'-cyclic phosphodiesterase,3.1.4.35 ,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE,PDE5A,PDE5,

Tissue Specificity: Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Background: cGMP-specific phosphodiesterase type 5 is an enzyme from the phosphodiesterase class. It is found in various tissues, most prominently the corpus cavernosum and the retina. It has also been recently discovered to play a vital role in the cardiovascular system. Furthermore, PDE5A also plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This PDE5A gene is mapped to 4q26.

Molecular Weight: 100 kDa

Gene ID: 8654

UniProt: O76074

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling