TNFRSF10B antibody (AA 233-440)

Catalog No. ABIN5518794

This Rabbit Polyclonal antibody specifically detects TNFRSF10B in WB, FACS and IHC. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for TNFRSF10B antibody (AA 233-440) (ABIN5518794)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF10B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-TNFRSF10B Antibody

Binding Specificity: AA 233-440

Purpose: Anti-DR5/TNFRSF10B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DR5/TNFRSF10B Antibody Picoband® (ABIN5518794). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DR5 recombinant protein (Position: K233-S440). Human DR5 shares 48.4% amino acid (aa) sequence identity with mouse DR5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Finnberg, N., Klein-Szanto, A. J. P., El-Deiry, W. S.TRAIL-R deficiency in mice promotes susceptibility to chronic inflammation and tumorigenesis.J. Clin. Invest. 118: 111-123, 2008. 2. Takeda, K., Kojima, Y., Ikejima, K., Harada, K., Yamashina, S., Okumura, K., Aoyama, T., Frese, S., Ikeda, H., Haynes, N. M., Cretney, E., Yagita, H., Sueyoshi, N., Sato, N., Nakanuma, Y., Smyth, M. J., Okumura, K.Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease.Proc. Nat. Acad. Sci. 105: 10895-10900, 2008. 3. Walczak, H., Degli-Esposti, M. A., Johnson, R. S., Smolak, P. J., Waugh, J. Y., Boiani, N., Timour, M. S., Gerhart, M. J., Schooley, K. A., Smith, C. A., Goodwin, R. G., Rauch, C. T.TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL.EMBO J. 16: 5386-5397, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TNFRSF10B antibody (ABIN5518794)

Liu, Zuo, Wang, Chen, Yang, Wang, Zhu: "miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT." in: Oncotarget, Vol. 5, Issue 13, pp. 4959-71, (2014) (PubMed).

Target Details for TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TNFRSF10B

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 10B,Death receptor 5,TNF-related apoptosis-inducing ligand receptor 2,TRAIL receptor 2,TRAIL-R2,CD262,TNFRSF10B,DR5, KILLER, TRAILR2, TRICK2, ZTNFR9,UNQ160/PRO186,

Tissue Specificity: Widely expressed in adult and fetal tissues, very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361, highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract, not detectable in brain.

Background: TNFRSF10B (Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, this gene is mapped to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.

Molecular Weight: 60 kDa

Gene ID: 8795

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity