AMH antibody (AA 75-141)

Catalog No. ABIN5518803

This Rabbit Polyclonal antibody specifically detects AMH in WB. It exhibits reactivity toward Human.

Quick Overview for AMH antibody (AA 75-141) (ABIN5518803)

Target: AMH (Anti-Mullerian Hormone (AMH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AMH Antibody

Binding Specificity: AA 75-141

Purpose: Anti-AMH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AMH Antibody Picoband® (ABIN5518803). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AMH recombinant protein (Position: A75-E141). Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Behringer RR (1994). "The in vivo roles of müllerian-inhibiting substance". Current Topics in Developmental Biology. Current Topics in Developmental Biology. 29: 171-87. 2. Cate RL, Mattaliano RJ, Hession C, Tizard R, Farber NM, Cheung A, Ninfa EG, Frey AZ, Gash DJ, Chow EP (Jun 1986). "Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells". Cell. 45 (5): 685-98. 3. Taguchi O, Cunha GR, Lawrence WD, Robboy SJ (Dec 1984). "Timing and irreversibility of Müllerian duct inhibition in the embryonic reproductive tract of the human male". Developmental Biology. 106 (2): 394-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AMH

Target: AMH (Anti-Mullerian Hormone (AMH))

Alternative Name: AMH

Background:

Synonyms: Muellerian-inhibiting factor,Anti-Muellerian hormone,AMH,Muellerian-inhibiting substance,MIS,AMH,MIF,

Tissue Specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.

Background: Anti-Müllerian hormone (AMH), also known as MIF or MIS, is a protein that in humans is encoded by the AMH gene. It is a hormone that inhibits the development of the Müllerian ducts (paramesonephric ducts) in the male embryo. Expression of AMH is activated by SOX9 in the male Sertoli cells and causes the irreversible regression of the Müllerian ducts. Because AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome.

Molecular Weight: 65 kDa

Gene ID: 268

UniProt: P03971

Pathways: Negative Regulation of Hormone Secretion