AMY1A antibody (N-Term)

Catalog No. ABIN5518804

The Rabbit Polyclonal anti-AMY1A antibody has been validated for WB and IHC. It is suitable to detect AMY1A in samples from Human, Mouse and Rat.

Quick Overview for AMY1A antibody (N-Term) (ABIN5518804)

Target: AMY1A (Amylase, alpha 1A (Salivary) (AMY1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMY1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AMY1A Antibody

Binding Specificity: AA 20-50, N-Term

Purpose: Anti-Amylase/AMY1A/AMY1B/AMY1C Antibody Picoband®

Sequence: NTQQGRTSIV HLFEWRWVDI ALECERYLAP K

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Amylase/AMY1A/AMY1B/AMY1C Antibody Picoband® (ABIN5518804). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Dracopoli, N. C., Meisler, M. H.Mapping the human amylase gene cluster on the proximal short arm of chromosome 1 using a highly informative (CA)n repeat.Genomics 7: 97-102, 1990. 2. Gumucio, D. L., Wiebauer, K., Caldwell, R. M., Samuelson, L. C., Meisler, M. H.Concerted evolution of human amylase genes.Molec. Cell. Biol. 8: 1197-1205, 1988. 3. Kamaryt, J., Laxova, R.Amylase heterogeneity: some genetic and clinical aspects.Humangenetik 1: 579-586, 1965.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AMY1A

Target: AMY1A (Amylase, alpha 1A (Salivary) (AMY1A))

Alternative Name: AMY1A

Background:

Synonyms: Alpha-amylase 1,3.2.1.1,1,4-alpha-D-glucan glucanohydrolase 1,Salivary alpha-amylase,AMY1A,AMY1,AMY1B,AMY1,AMY1C,AMY1,

Tissue Specificity: Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Expressed in the lens, cornea and retina. Strongly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body.

Background: Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21. Amylase enzymes find use in bread making and to break down complex sugars such as starch (found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.

Molecular Weight: 58 kDa

Gene ID: 276, 277, 278

UniProt: P04745