ARNTL antibody (AA 58-156)

Catalog No. ABIN5518808

The Rabbit Polyclonal anti-ARNTL antibody (ABIN5518808) specifically detects ARNTL in WB and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ARNTL antibody (AA 58-156) (ABIN5518808)

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARNTL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ARNTL Antibody

Binding Specificity: AA 58-156

Purpose: Anti-BMAL1/ARNTL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BMAL1/ARNTL Antibody (ABIN5518808). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BMAL1/ARNTL recombinant protein (Position: H58-D156). Human BMAL1/ARNTL shares 99% and 98% amino acid (aa) sequence identity with mouse and rat BMAL1/ARNTL, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Alternative Name: ARNTL

Background:

Synonyms: Aryl hydrocarbon receptor nuclear translocator-like protein 1,Basic-helix-loop-helix-PAS protein MOP3,Brain and muscle ARNT-like 1,Class E basic helix-loop-helix protein 5,bHLHe5,Member of PAS protein 3,PAS domain-containing protein 3,bHLH-PAS protein JAP3,ARNTL,BHLHE5, BMAL1, MOP3, PASD3,

Tissue Specificity: Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart. .

Background: Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 75 kDa

Gene ID: 406

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect