ATF6 antibody (C-Term)

Catalog No. ABIN5518809

This Rabbit Polyclonal antibody specifically detects ATF6 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATF6 antibody (C-Term) (ABIN5518809)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATF6 Antibody

Binding Specificity: AA 597-629, C-Term

Purpose: Anti-ATF6 Antibody Picoband®

Sequence: AININENVIN GQDYEVMMQI DCQVMDTRIL HIK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ATF6 Antibody Picoband® (ABIN5518809). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ATF6, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Li, M., Baumeister, P., Roy, B., Phan, T., Foti, D., Luo, S., Lee, A. S. : ATF6 as a transcription activator of the endoplasmic reticulum stress element: thapsigargin stress-induced changes and synergistic interactions with NF-Y and YY1. Molec. Cell. Biol. 20: 5096-5106, 2000. 2. Thameem, F., Farook, V. S., Bogardus, C., Prochazka, M. : Association of amino acid variants in the activating transcription factor 6 gene (ATF6) on 1q21-q23 with type 2 diabetes in Pima Indians. Diabetes 55: 839-842, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6

Background:

Synonyms: Cyclic AMP-dependent transcription factor ATF-6 alpha,cAMP-dependent transcription factor ATF-6 alpha,Activating transcription factor 6 alpha,ATF6-alpha,Processed cyclic AMP-dependent transcription factor ATF-6 alpha,ATF6,

Tissue Specificity: Ubiquitous.

Background: ATF6, a member of the leucine zipper protein family, is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules. This gene is mapped to chromosome 1q23.3. ATF6 can constitutively induce the promoter of glucose-regulated protein (grp) genes through activation of the endoplasmic reticulum (ER) stress element (ERSE).

Molecular Weight: 90 kDa, 100 kDa

Gene ID: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response