BAG5 antibody (AA 389-447)

Catalog No. ABIN5518812

This Rabbit Polyclonal antibody specifically detects BAG5 in WB, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BAG5 antibody (AA 389-447) (ABIN5518812)

Target: BAG5 (BCL2-Associated Athanogene 5 (BAG5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-BAG5 Antibody

Binding Specificity: AA 389-447

Purpose: Anti-Bag5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Bag5 Antibody Picoband® (ABIN5518812). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Takayama S, Xie Z, Reed JC (Feb 1999). "An evolutionarily conserved family of Hsp70/Hsc70 Molecular chaperone regulators". J Biol Chem 274 (2): 781-6. 2. Kalia SK, Lee S, Smith PD, Liu L, Crocker SJ, Thorarinsdottir TE, Glover JR, Fon EA, Park DS, Lozano AM (Dec 2004). "BAG5 inhibits parkin and enhances dopaminergic neuron degeneration". Neuron 44 (6): 931-45. 3. "Entrez Gene: BAG5 BCL2-associated athanogene 5".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BAG5

Target: BAG5 (BCL2-Associated Athanogene 5 (BAG5))

Alternative Name: BAG5

Background:

Synonyms: BAG family molecular chaperone regulator 5,BAG-5,Bcl-2-associated athanogene 5,BAG5,KIAA0873,

Tissue Specificity: Expressed in all tissues examined with lower levels in brain and testis.

Background: BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. It is mapped to 14q32.33. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.

Molecular Weight: 51 kDa

Gene ID: 9529

Pathways: SARS-CoV-2 Protein Interactome