Survivin antibody (AA 1-142)

Catalog No. ABIN5518813

The Rabbit Polyclonal anti-Survivin antibody has been validated for WB, ELISA and IHC. It is suitable to detect Survivin in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for Survivin antibody (AA 1-142) (ABIN5518813)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Survivin Antibody

Binding Specificity: AA 1-142

Purpose: Anti-Survivin/BIRC5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Survivin/BIRC5 Antibody Picoband® (ABIN5518813). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Survivin recombinant protein (Position: M1-D142). Human Survivin shares 84.3% and 83% amino acid (aa) sequence identity with mouse and rat Survivin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Ambrosini, G., Adida, C., Altieri, D. C.A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.Nature Med. 3: 917-921, 1997. 2. Chantalat, L., Skoufias, D. A., Kleman, J.-P., Jung, B., Dideberg, O., Margolis, R. L.Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions.Molec. Cell 6: 183-189, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Survivin antibody (ABIN5518813)

Gu, Ren, Wang, Qu, Zhang: "Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance." in: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 14034-9, (2016) (PubMed).

Zhang, Wang, Yang, Pan, Li, Yuan: "Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing." in: Oncology letters, Vol. 11, Issue 5, pp. 3265-3272, (2016) (PubMed).

Chen, Xie, Zhao, Wang, Bai, Yin, Jiang, Xie, Jia, Huang et al.: "Ampelopsin induces apoptosis by regulating multiple c?Myc/S?phase kinase?associated protein 2/F?box and WD repeat?containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma ..." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 105-12, (2014) (PubMed).

Cai, Liu, Zhao, Wan, Hu, Zhou, Chen: "Expressions of survivin and vascular endothelial growth factor in a Murine model of proliferative retinopathy." in: International journal of ophthalmology, Vol. 5, Issue 3, pp. 293-6, (2012) (PubMed).

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: BIRC5

Background:

Synonyms: Baculoviral IAP repeat-containing protein 5,Apoptosis inhibitor 4,Apoptosis inhibitor survivin,BIRC5,API4, IAP4,

Tissue Specificity: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. .

Background: Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. And this protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Molecular Weight: 16 kDa

Gene ID: 332

UniProt: O15392

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding