CRP antibody (AA 20-225)

Catalog No. ABIN5518820

Product Details anti-CRP Antibody

Target: CRP (C-Reactive Protein (CRP))

Binding Specificity: AA 20-225

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for C-reactive protein(Crp) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for C-reactive protein(Crp) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: C-reactive protein, pentraxin-related
Protein Name: C-reactive protein

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Crp recombinant protein (Position: H20-S225). Mouse Crp shares 71.9% and 72.7% amino acid (aa) sequence identity with human and rat Crp, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive Protein (CRP Products)

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Synonyms: C-reactive protein | C-reactive protein(1-205) | CRP | PTX1 | P02741

Gene ID: 12944

UniProt: P14847

Pathways: Carbohydrate Homeostasis