EIF3E antibody (AA 160-241)

Catalog No. ABIN5518829

This anti-EIF3E antibody is a Rabbit Polyclonal antibody detecting EIF3E in WB. Suitable for Human, Mouse and Rat.

Quick Overview for EIF3E antibody (AA 160-241) (ABIN5518829)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3E Antibody

Binding Specificity: AA 160-241

Purpose: Anti-EIF3e Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EIF3e Antibody Picoband® (ABIN5518829). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF3e recombinant protein (Position: A160-Q241). Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Asano K, Merrick WC, Hershey JW (October 1997). "The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome". J. Biol. Chem. 272 (38): 23477-80. 2. Miyazaki, S., Imatani, A., Ballard, L., Marchetti, A., Buttitta, F., Albertsen, H., Nevanlinna, H. A., Gallahan, D., Callahan, R. The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. Genomics 46: 155-158, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit E ,eIF3e ,Eukaryotic translation initiation factor 3 subunit 6 ,Viral integration site protein INT-6 homolog,eIF-3 p48 ,EIF3E ,EIF3S6 , INT6 ,

Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. .

Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 52 kDa

Gene ID: 3646

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C