STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (AA 2-230)

Details for Product anti-STAT1 Antibody No. ABIN5518871
Target
Binding Specificity
AA 2-230
50
37
29
17
15
15
8
8
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
293
160
155
24
24
23
20
18
13
10
8
6
5
5
2
1
1
1
1
1
Host
Rabbit
237
63
2
2
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
15
11
11
10
9
9
9
8
8
5
5
4
4
4
4
4
3
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
207
96
88
60
55
47
23
22
11
8
4
4
4
1
1
Options
Purpose Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 1-alpha/beta(STAT1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 1-alpha/beta(STAT1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: signal transducer and activator of transcription 1, 91 kDa
Protein Name: Signal transducer and activator of transcription 1-alpha/beta
Purification Immunogen affinity purified.
Target
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Synonyms: Signal transducer and activator of transcription 1-alpha/beta | Transcription factor ISGF-3 components p91/p84 | STAT1 | STAT 1 | P42224
Gene ID 6772
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Images
Image no. 1 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 2-230) antibody (ABIN5518871) Western blot analysis of STAT1 using anti-STAT1 antibody . Electrophoresis was perfo...
Image no. 2 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 2-230) antibody (ABIN5518871) IHC analysis of STAT1 using anti- STAT1 antibody . STAT1 was detected in paraffin-emb...
Product cited in: Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, 2018 (PubMed).

Gong, Cao, Jiang, Zhou, Liu: "Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation." in: World journal of gastroenterology, Vol. 13, Issue 30, pp. 4080-4, 2007 (PubMed).