STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701)

Details for Product anti-STAT1 Antibody No. ABIN6255991
Target
Binding Specificity
pTyr701
50
36
29
17
15
15
8
8
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
293
160
155
24
24
23
20
18
13
10
8
6
5
5
2
1
1
1
1
1
Host
Rabbit
237
63
2
2
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
15
11
11
10
9
9
9
8
8
5
5
4
4
4
4
4
3
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
207
95
87
59
55
48
22
22
10
8
4
4
4
1
1
Options
Immunogen A synthesized peptide derived from human STAT1 around the phosphorylation site of Tyrosine 701
Isotype IgG
Specificity Phospho-STAT1 (Tyr701) Antibody detects endogenous levels of STAT1 only when phosphorylated at Tyrosine 701
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene: STAT1

Molecular Weight 84kDa
Gene ID 6772
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IP, IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Images
Image no. 1 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (ABIN6255991) ABIN6267509 at 1/200 staining Mouse pancreas tissue sections by IHC-P. The tissue was...
Image no. 2 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (ABIN6255991) Western blot analysis of STAT1 phosphorylation expression in MCF7 whole cell lysates,...
Image no. 3 for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (ABIN6255991) ABIN6267509 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Product cited in: Liu, Wu, Zhu, Li, Liu: "IRG1 increases MHC class I level in macrophages through STAT-TAP1 axis depending on NADPH oxidase mediated reactive oxygen species." in: International immunopharmacology, Vol. 48, pp. 76-83, 2018 (PubMed).

Chen, Zhou, Liu, Huang, Liu, Kang, Chen, Guo, Zhu, Sun: "Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway." in: Oncology reports, Vol. 39, Issue 3, pp. 1081-1089, 2018 (PubMed).