STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727)

Details for Product anti-STAT1 Antibody No. ABIN6256410
Antigen
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Alternatives
anti-Human STAT1 antibody for Immunoprecipitation
Epitope
pSer727
101
75
51
17
17
15
9
8
8
8
7
7
6
6
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
552
302
269
33
29
26
22
22
16
13
11
7
7
5
2
2
2
1
1
1
1
1
Host
Rabbit
475
106
2
1
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
19
15
12
10
10
10
10
10
10
6
6
4
4
4
4
4
4
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
449
208
164
109
90
52
47
17
17
9
7
6
5
3
1
1
Options
Immunogen A synthesized peptide derived from human STAT1 around the phosphorylation site of Serine 727
Isotype IgG
Specificity Phospho-STAT1 (Ser727) Antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Plasmids, Primers & others Plasmids, Primers & others STAT1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene: STAT1

Molecular Weight 91kDa
Gene ID 6772
UniProt P42224
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) Western blot analysis of STAT1 phosphorylation expression in Jurkat whole cell lysate...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Human bladder cancer tissue sections by IHC-P. The tiss...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was for...
Immunohistochemistry (IHC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was forma...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (ABIN6256410) ABIN6267508 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized...
Product cited in: Wu, Chen, Jing, Gu, Mei, Yao, Zhou, Yang, Sun, Wang, Hu, Wüthrich, Mei: "The C-terminal tail of polycystin-1 regulates complement factor B expression by signal transducer and activator of transcription 1." in: American journal of physiology. Renal physiology, Vol. 310, Issue 11, pp. F1284-94, 2017 (PubMed).

Did you look for something else?