Caspase 6 antibody (AA 194-293)

Catalog No. ABIN5518898

This anti-Caspase 6 antibody is a Rabbit Polyclonal antibody detecting Caspase 6 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for Caspase 6 antibody (AA 194-293) (ABIN5518898)

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 6 Antibody

Binding Specificity: AA 194-293

Purpose: Anti-Caspase-6/CASP6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Caspase-6/CASP6 Antibody Picoband® (ABIN5518898). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Caspase-6 recombinant protein (Position: A194-N293). Human Caspase-6 shares 95.9% and 93.9% amino acid (aa) sequence identity with mouse and rat Caspase-6, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Fernandes-Alnemri, T., Litwack, G., Alnemri, E. S.Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family.Cancer Res. 55: 2737-2742, 1995. 2. Nikolaev, A., McLaughlin, T., O'Leary, D. D. M., Tessier-Lavigne, M.APP binds DR6 to trigger axon pruning and neuron death via distinct caspases Nature 457: 981-989, 2009. 3. Orth, K., Chinnaiyan, A. M., Garg, M., Froelich, C. J., Dixit, V. M.The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A.J. Biol. Chem. 271: 16443-16446, 1996.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: CASP6

Background:

Synonyms: Caspase-6,CASP-6,3.4.22.59,Apoptotic protease Mch-2,Caspase-6 subunit p18,Caspase-6 subunit p11,CASP6,MCH2,

Tissue Specificity: Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.

Background: Caspase 6 is an enzyme that in humans is encoded by the CASP6 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Using radiation hybrid mapping, the CASP6 gene is localized to human chromosome 4q25-q26. It functions as a downstream enzyme in the caspase activation cascade. And CASP6 can cleave lamin A to its signature apoptotic fragment.

Molecular Weight: 33 kDa

Gene ID: 839

UniProt: P55212

Pathways: Apoptosis, Caspase Cascade in Apoptosis