DDX58 antibody (AA 871-925)

Catalog No. ABIN5518906

Product Details anti-DDX58 Antibody

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Binding Specificity: AA 871-925

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX58 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX58(DDX58) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX58(DDX58) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: DExD/H-box helicase 58
Protein Name: Probable ATP-dependent RNA helicase DDX58

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human DDX58 recombinant protein (Position: H871-K925). Human DDX58 shares 83.3% amino acid (aa) sequence identity with mouse DDX58.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: DDX58 (DDX58 Products)

Synonyms: RIG-I antibody, RIGI antibody, RLR-1 antibody, 6430573D20Rik antibody, C330021E21 antibody, RHIV-1 antibody, RIG-1 antibody, DExD/H-box helicase 58 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 antibody, DEXD/H-box helicase 58 antibody, DDX58 antibody, Ddx58 antibody

Background: RIG-I (retinoic acid-inducible gene I) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded (in humans) by the DDX58 gene. RIG-I is part of the RIG-I-like receptor family, which also includes MDA5 and LGP2, and functions as a pattern recognition receptor that is a sensor for viruses such as influenza A, Sendai virus, and flavivirus. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Synonyms: Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I, DDX58

Gene ID: 23586

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C