EGF antibody (AA 974-1026)

Catalog No. ABIN5518908

This Rabbit Polyclonal antibody specifically detects EGF in WB, IHC and ELISA. It exhibits reactivity toward Rat and has been mentioned in 2+ publications.

Quick Overview for EGF antibody (AA 974-1026) (ABIN5518908)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EGF Antibody

Binding Specificity: AA 974-1026

Purpose: Anti-EGF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EGF Antibody Picoband® (ABIN5518908). Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat
ELISA(Cap) , 1-5 μg/mL, Rat
1. Brissenden, J. E., Ullrich, A., Francke, U.Chromosomal mapping of loci for insulin-like growth factors I and II and for epidermal growth factor in man.Am. J. Hum. Genet.36: 133S-only, 1984. 2. Carpenter G, Cohen S (May 1990). "Epidermal growth factor". The Journal of Biological Chemistry 265 (14): 7709-12. 3. Herbst RS (2004). "Review of epidermal growth factor receptor biology". International Journal of Radiation Oncology, Biology, Physics 59 (2 Suppl): 21-6.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-EGF antibody (ABIN5518908)

Wang, Li, Fu, Li, Yang, Zhang, Zhu, Yang, Gu, Xing, Zhang: "Exemestane Attenuates Hepatic Fibrosis in Rats by Inhibiting Activation of Hepatic Stellate Cells and Promoting the Secretion of Interleukin 10." in: Journal of immunology research, Vol. 2017, pp. 3072745, (2018) (PubMed).

Messini: "[Therapy of total arrhythmia due to atrial fibrillation and flutter]." in: La Clinica terapeutica, Vol. 57, Issue 2, pp. 109-28, (1971) (PubMed).

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Egf

Background:

Synonyms: Pro-epidermal growth factor,EGF,Epidermal growth factor,Egf,

Tissue Specificity: B-cells. Expressed in a number of B-cell lines including P32/ISH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

Background: EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Molecular Weight: 6 kDa

Gene ID: 25313

UniProt: P07522

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation