MAVS antibody (AA 34-96)

Catalog No. ABIN5518935

This Rabbit Polyclonal antibody specifically detects MAVS in WB, IHC, ICC, IHC (fro) and FACS. It exhibits reactivity toward Human.

Quick Overview for MAVS antibody (AA 34-96) (ABIN5518935)

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAVS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Product Details anti-MAVS Antibody

Binding Specificity: AA 34-96

Purpose: Anti-MAVS Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAVS Antibody Picoband® (ABIN5518935). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MAVS recombinant protein (Position: L34-Q96). Human MAVS shares 72.6% and 69.4% amino acid (aa) sequence identity with mouse and rat MAVS, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human,
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Seth RB, Sun L, Ea CK, Chen ZJ (Sep 2005). "Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3". Cell. 122 (5): 669-82. 2. Xu LG, Wang YY, Han KJ, Li LY, Zhai Z, Shu HB (Sep 2005). "VISA is an adapter protein required for virus-triggered IFN-beta signaling". Mol Cell. 19 (6): 727-40.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAVS

Target: MAVS (Mitochondrial Antiviral Signaling Protein (MAVS))

Alternative Name: MAVS

Background:

Synonyms: Mitochondrial antiviral-signaling protein,MAVS,CARD adapter inducing interferon beta,Cardif,Interferon beta promoter stimulator protein 1,IPS-1,Putative NF-kappa-B-activating protein 031N,Virus-induced-signaling adapter,VISA,MAVS,IPS1, KIAA1271, VISA,

Tissue Specificity: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. .

Background: Mitochondrial antiviral-signaling protein (MAVS) is a protein that in humans is encoded by the MAVS gene. The protein is also known by the names VISA (virus-induced signaling adapter), IPS-1 and Cardif. This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity.

Molecular Weight: 75 kDa

Gene ID: 57506

UniProt: Q7Z434

Pathways: Activation of Innate immune Response, Inositol Metabolic Process, Hepatitis C