Myoglobin antibody (N-Term)

Catalog No. ABIN5518936

The Rabbit Polyclonal anti-Myoglobin antibody is suitable to detect Myoglobin in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for Myoglobin antibody (N-Term) (ABIN5518936)

Target: Myoglobin (MB)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myoglobin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Myoglobin Antibody

Binding Specificity: N-Term

Purpose: Anti-Myoglobin/MB Antibody

Sequence: LSDGEWQLVL NVWGKVEADI PGHGQEVLIR LFK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by three amino acids,and from the related rat sequence by six amino acids.

Characteristics: Anti-Myoglobin/MB Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin, different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Myoglobin

Target: Myoglobin (MB)

Alternative Name: Myoglobin

Background:

Background: Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.

Gene Full Name: myoglobin

Molecular Weight: 17 kDa

Gene ID: 4151

UniProt: P02144

Pathways: Brown Fat Cell Differentiation