Phenylalanine Hydroxylase antibody (AA 71-208)

Catalog No. ABIN5518942

Product Details anti-Phenylalanine Hydroxylase Antibody

Target: Phenylalanine Hydroxylase

Binding Specificity: AA 71-208

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phenylalanine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Phenylalanine-4-hydroxylase(PAH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Phenylalanine-4-hydroxylase(PAH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: phenylalanine hydroxylase
Protein Name: Phenylalanine-4-hydroxylase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Alternative Name: PAH (Phenylalanine Hydroxylase Products)

Synonyms: cb877 antibody, wu:fb02f10 antibody, zgc:65981 antibody, GB18494 antibody, PAH antibody, pku antibody, pku1 antibody, DDBDRAFT_0206188 antibody, DDBDRAFT_0231664 antibody, DDB_0206188 antibody, DDB_0231664 antibody, AW106920 antibody, PH antibody, PKU antibody, PKU1 antibody, phenylalanine-4-hydroxylase antibody, phenylalanine hydroxylase antibody, protein henna antibody, phenylalanine 4-monooxygenase antibody, pah antibody, LOC408622 antibody, PAH antibody, CpipJ_CPIJ002149 antibody, Pah antibody

Target Type: Chemical

Background: Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Synonyms: Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase

Gene ID: 5053

UniProt: P00439