PREB antibody (AA 42-236)

Catalog No. ABIN5518945

The Rabbit Polyclonal anti-PREB antibody has been validated for WB. It is suitable to detect PREB in samples from Human.

Quick Overview for PREB antibody (AA 42-236) (ABIN5518945)

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PREB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PREB Antibody

Binding Specificity: AA 42-236

Purpose: Anti-PREB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PREB Antibody Picoband® (ABIN5518945). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PREB recombinant protein (Position: K42-N236). Human PREB shares 87.7% amino acid (aa) sequence identity with both mouse and rat PREB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Edgar AJ (Jul 2003). "The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12". BMC Genomics. 4: 18. 2. Fliss MS, Hinkle PM, Bancroft C (May 1999). "Expression cloning and characterization of PREB (prolactin regulatory element binding), a novel WD motif DNA-binding protein with a capacity to regulate prolactin promoter activity". MolEndocrinol. 13 (4): 644-657.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PREB

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Alternative Name: PREB

Background:

Synonyms: Prolactin regulatory element-binding protein,Mammalian guanine nucleotide exchange factor mSec12,PREB,SEC12,

Tissue Specificity: Ubiquitous. .

Background: Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene. This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.

Molecular Weight: 45 kDa

Gene ID: 10113

Pathways: ER-Nucleus Signaling