MED6 antibody (AA 40-246)

Catalog No. ABIN5519030

This anti-MED6 antibody is a Rabbit Polyclonal antibody detecting MED6 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for MED6 antibody (AA 40-246) (ABIN5519030)

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED6 Antibody

Binding Specificity: AA 40-246

Purpose: Anti-MED6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED6 Antibody Picoband® (ABIN5519030). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED6 recombinant protein (Position: F40-Q246).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD (Aug 1998). "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways". ProcNatlAcadSci U S A. 95 (15): 8538-8543. 2. Lee YC, Min S, Gim BS, Kim YJ (August 1997). "A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans". Mol. Cell. Biol. 17 (8): 4622-32.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MED6

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Alternative Name: MED6

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, ARC33, Mediator complex subunit 6, hMed6, Renal carcinoma antigen NY-REN-28, MED6, ARC33

Background: Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.

Molecular Weight: 32 kDa

Gene ID: 10001

UniProt: O75586

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha