MED9 antibody (AA 55-146)

Catalog No. ABIN5519032

The Rabbit Polyclonal anti-MED9 antibody has been validated for WB, ELISA, IF, FACS, ICC and IHC. It is suitable to detect MED9 in samples from Human, Mouse and Rat.

Quick Overview for MED9 antibody (AA 55-146) (ABIN5519032)

Target: MED9 (Mediator Complex Subunit 9 (MED9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Product Details anti-MED9 Antibody

Binding Specificity: AA 55-146

Purpose: Anti-MED9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MED9 Antibody Picoband® (ABIN5519032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED9 recombinant protein (Position: A55-E146).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Tomomori-Sato, C., Sato, S., Parmely, T. J., Banks, C. A. S., Sorokina, I., Florens, L., Zybailov, B., Washburn, M. P., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. J. Biol. Chem. 279: 5846-5851, 2004.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MED9

Target: MED9 (Mediator Complex Subunit 9 (MED9))

Alternative Name: MED9

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on chromosome 17.

Molecular Weight: 19 kDa

Gene ID: 55090

Pathways: Regulation of Lipid Metabolism by PPARalpha