SAG antibody (AA 193-405)

Catalog No. ABIN5519058

This Rabbit Polyclonal antibody specifically detects SAG in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SAG antibody (AA 193-405) (ABIN5519058)

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SAG Antibody

Binding Specificity: AA 193-405

Purpose: Anti-Retinal S antigen/SAG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Retinal S antigen/SAG Antibody Picoband® (ABIN5519058). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Retinal S antigen recombinant protein (Position: R193-E405).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Calabrese, G., Sallese, M., Stornaiuolo, A., Stuppia, L., Palka, G., De Blasi, A. Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes. Genomics 23: 286-288, 1994. 2. Yamaki, K., Tsuda, M., Kikuchi, T., Chen, K.-H., Huang, K.-P., Shinohara, T. Structural organization of the human S-antigen gene: cDNA, amino acid, intron, exon, promoter, in vitro transcription, retina, and pineal gland. J. Biol. Chem. 265: 20757-20762, 1990.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SAG

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Alternative Name: SAG

Background:

Synonyms: S-arrestin, 48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin, SAG,

Tissue Specificity: Retina and pineal gland.

Background: S-arrestin is a protein that in humans is encoded by the SAG gene. Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness.

Molecular Weight: 45-55 kDa

Gene ID: 6295

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling