TREM1 antibody (AA 21-200)

Catalog No. ABIN5519068

There is 1 publication for this product available. The Rabbit Polyclonal anti-TREM1 antibody is suitable to detect TREM1 in samples from Human. It has been validated for WB, FACS, IHC and ICC.

Quick Overview for TREM1 antibody (AA 21-200) (ABIN5519068)

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-TREM1 Antibody

Binding Specificity: AA 21-200

Purpose: Anti-TREM1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TREM1 Antibody. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TREM1 recombinant protein (Position: A21-R200).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry, 0.5-1 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-TREM1 antibody (ABIN5519068)

Zhou, Xiong, Huang, Tang, Yu, Lan: "Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis." in: Scientific reports, Vol. 5, pp. 17901, (2016) (PubMed).

Target Details for TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Alternative Name: TREM1

Background:

Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Gene Full Name: triggering receptor expressed on myeloid cells 1

Molecular Weight: 26 kDa

Gene ID: 54210

UniProt: Q9NP99