TREM1 antibody (AA 21-202)

Catalog No. ABIN5519069

Cited in 1 publication. The Rabbit Polyclonal anti-TREM1 antibody (ABIN5519069) specifically detects TREM1 in WB and ELISA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for TREM1 antibody (AA 21-202) (ABIN5519069)

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TREM1 Antibody

Binding Specificity: AA 21-202

Purpose: Anti-TREM1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TREM1 Antibody (ABIN5519069). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse TREM1 recombinant protein (Position: A21-S202).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA (Cap), 1-5 μg/mL

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TREM1 antibody (ABIN5519069)

Zhou, Xiong, Huang, Tang, Yu, Lan: "Identification of Genes Associated with Smad3-dependent Renal Injury by RNA-seq-based Transcriptome Analysis." in: Scientific reports, Vol. 5, pp. 17901, (2016) (PubMed).

Target Details for TREM1

Target: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Alternative Name: Trem1

Background:

Synonyms: Triggering receptor expressed on myeloid cells 1, TREM-1, CD354, Trem1,

Background: Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF), MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Molecular Weight: 26 kDa

Gene ID: 58217