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Chiffon antibody (C-Term)

CHIF Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5530173
  • Target See all Chiffon (CHIF) products
    Chiffon (CHIF)
    Binding Specificity
    AA 476-504, C-Term
    Reactivity
    Human
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This Chiffon antibody is un-conjugated
    Application
    Western Blotting (WB)
    Purification
    This antibody is purified through a protein A column, followed by peptide affinity purification.
    Immunogen
    This CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CHFR.
    Isotype
    Ig Fraction
  • Application Notes
    For WB starting dilution is: 1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    Chiffon (CHIF)
    Alternative Name
    CHF (CHIF Products)
    Background
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
    Molecular Weight
    73 kDa
    Gene ID
    55743
    UniProt
    Q96EP1
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