HCK antibody (N-Term)

Catalog No. ABIN5531324

This Rabbit Polyclonal antibody specifically detects HCK in WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HCK antibody (N-Term) (ABIN5531324)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HCK Antibody

Binding Specificity: AA 131-156, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from the N-terminal region of human HCK.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: HCK

Background: HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq].

Molecular Weight: 60 kDa

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling