RPA1 antibody (C-Term)

Catalog No. ABIN5532052

This anti-RPA1 antibody is a Rabbit Polyclonal antibody detecting RPA1 in WB. Suitable for Human.

Quick Overview for RPA1 antibody (C-Term) (ABIN5532052)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPA1 Antibody

Binding Specificity: AA 531-559, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-559 amino acids from the C-terminal region of human RPA1.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1

Background: RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Molecular Weight: 68 kDa

Gene ID: 6117

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA